Next Generation Sequencing Data Analysis
Bioinformatics core facility
Ben-Gurion University of the Negev
The revolution in sequencing technology has made the generation of large quantities of sequences available and affordable.
Among the popular Next Generation Sequencing applications are DNA-Seq for genome studies, RNA-Seq for transcriptome studies, Chip-Seq for protein-DNA interactions and CNV-Seq for large genome nucleotide variations.
Nowadays, more and more scientists not only conduct their own sequencing projects, but many of them also benefit from the huge amounts of publicly available data.
In this workshop we encourage participants to start "wetting their hands" in data retrieval, analysis and visualization, using freely available, user-friendly tools.
We will start by presenting the common standard formats for representing sequence data (FASTQ) and sequence alignment & mapping results (SAM).
Subsequently, we will learn how to handle the Next Generation Sequencing data and extract information of interest using
Galaxy, and then we will visualize it in an easy manner with the
Integrative Genomics Viewer (IGV).
We hope that by the end of the workshop, participants will be equipped not only with theoretical, but also with practical knowledge of how to start working with data produced by the latest sequencing technology.
The workshop will be delivered in Hebrew.
No prior programming experience is required.
About the lecturer:
Ilia Zhidkov has experience in Next Generation Sequencing data analysis, specifically with characterization of human mitochondrial DNA.
In 2010, he participated in a Cold Spring Harbor Laboratories, course: Advanced Sequencing Technologies & Applications.
He now works at the NIBN bioinformatics core facility in Ben-Gurion university of the Negev, as coordinator of Next Generation Sequencing projects.
Tuesday 17.5.2011, 8:15 - 14:00
#306 PC classroom, 2nd floor of the Library building
275 NIS per participant paid to the NIBN, BGU